Steve R. Posted May 26, 2015 Report Share Posted May 26, 2015 Identifying the species by performing a genetic test is pretty straight forward. Usually you amplify a highly conserved gene (often a gene coding for ribosomal RNA), sequence it and compare it to the available sequences. This is actually what I want to do with my students next Fall in the Molecular Genetics Lab. class that I am teaching. I estimate that the cost for us will be about $20 per sample (costs for materials and sequencing). Soothing Shrimp and JamesHe 2 Quote Link to comment Share on other sites More sharing options...
TheGardenofEder Posted May 26, 2015 Author Report Share Posted May 26, 2015 If this is all just becoming available how did breeders first get prls? Quote Link to comment Share on other sites More sharing options...
Steve R. Posted May 26, 2015 Report Share Posted May 26, 2015 PRL is a phenotypic (observable traits) definition. It means you only get red offspring, no blacks no snow whites. You have to have bred them form many generations. It is not a genetic definition. Some PRL's have also very intense white and good red coverage. Quote Link to comment Share on other sites More sharing options...
TheGardenofEder Posted May 26, 2015 Author Report Share Posted May 26, 2015 Well then guess I'll start a log on my generations and offspring but you would think if it wasn't prl 1 out of 100 would be cbs or golden at least right? Quote Link to comment Share on other sites More sharing options...
OMG Aquatics Posted May 26, 2015 Report Share Posted May 26, 2015 Well then guess I'll start a log on my generations and offspring but you would think if it wasn't prl 1 out of 100 would be cbs or golden at least right? http://www.silaneshrimp.com/ I assume you could do the same testing with cbs? Quote Link to comment Share on other sites More sharing options...
JamesHe Posted May 26, 2015 Report Share Posted May 26, 2015 Well then guess I'll start a log on my generations and offspring but you would think if it wasn't prl 1 out of 100 would be cbs or golden at least right? We had a good discussion on FB. I repost it here: What is the definition of Pure Line (Black or Red) in USA? I mean that the PRL or PBL means no King Kong offspring in Taiwan, but have chance to get golden shrimp (if mosura X mosura many times) . And it seems the definition of Pure Line is not all the same in other countries. Unlike · Comment · Share You, Eric Martens, Sami Mujahed and 3 others like this. Sami Mujahed . May 21 at 2:18am · Like Steve Barbee Prl x prl only get prl no golden or CBS or tb. all outcome PRL May 21 at 3:15am · Unlike · 4 Scott Bahr +1 to Steve May 21 at 3:24am · Unlike · 3 Joe Chang if PRL means no golden offspring, how come snow white can be breed? May 21 at 8:18am · Like Chris Li It takes years to clean out the golden genes. May 21 at 8:59am · Like · 1 James He +2 to Steve Barbee May 21 at 1:07pm · Like James He Snow White is not PRL. period. May 21 at 1:08pm · Edited · Like James He Chris Li Once it mixes with Golden, it would never be PRL. May 21 at 1:10pm · Edited · Like · 1 Sami Mujahed How many generations do u have to go on with only red to say it's pure? May 21 at 1:10pm · Like James He You can only create new PRL from PRL parents. May 21 at 1:11pm · Like · 3 James He Sami Mujahed: Never. May 21 at 1:12pm · Like · 2 Sami Mujahed gasp emoticon why? How.prl was created in the first place? Wasnt it selection? May 21 at 1:13pm · Like Sami Mujahed If u always take the tbs and golden out...at some point..u will have only red May 21 at 1:14pm · Like James He PRL started from the original CRS never crossbreed with golden. May 21 at 1:14pm · Like · 3 Sami Mujahed I ll debate u from another angle...if I have prl and breed it with golden...offsprings will have both genes... If they cross...some WILL be prl... Right? May 21 at 1:15pm · Like James He Sami, it's not worth to start the new PRL with unknow source. once you perfect the line and put it on market with your name. you don't want see your PRL produces golden offspring. it would ruin your reputation and hard work. May 21 at 1:17pm · Edited · Like · 3 James He Sami Mujahed how can you know if it carries golden gene or not? May 21 at 1:18pm · Like · 1 James He nobody knows what's golden gene really is. so you can't just decode the DNA to tell. May 21 at 1:20pm · Like · 2 Sami Mujahed Will a prl breeder have decoded DNA? Or his babies are pure red for many generations...? May 21 at 1:24pm · Like James He Sami no, it started from pure CRS line back 10 years ago. before the first golden showed up. that's the only way to guarantee it's pure. May 21 at 1:26pm · Edited · Like · 3 James He In reality, no all PRL meet the definition I mentioned above. so get PRL from reliable source is very important. like Ellen Wang line May 21 at 1:28pm · Edited · Like · 3 Steve Barbee James He has said it well and plus 1 to everything he said May 21 at 4:12pm · Like · 1 Joe Chang Do you guys ever let PRL-Mosura X PRL Mosura? PRL-Mosura X PRL Mosura will get PRL-flowerhead offspring. And if you let PRL-flowerhead X PRL-flowerhead , you will have chance to get golden. Can we say the PRL-flowerhead is not PRL? May 21 at 7:01pm · Like James He Joe, Is that your theory or you see it happened? Golden is mutation from CRS. it won't happen easily by breed PRL flowerhead together. and I didn't see lots of PRL flowerhead out there either. there is not recipe for golden Bee. it was mutation happened once in thousand or even millions of CRS. May 21 at 9:30pm · Edited · Like James He Most regular flowerhead was the result of cross breeding with golden in the past. so it has golden gene in it. May 21 at 9:31pm · Edited · Like Quote Link to comment Share on other sites More sharing options...
JamesHe Posted May 26, 2015 Report Share Posted May 26, 2015 I want to say one more thing. You can always create a new line of CRS. with good color and pattern you desire. Why you want to call it PRL? for example: Super Red Crystal, it's not necessary to be PRL to be qualified as good CRS. SRC is more expensive than regular PRL in the market. Quote Link to comment Share on other sites More sharing options...
TheGardenofEder Posted May 26, 2015 Author Report Share Posted May 26, 2015 I always like to breed with a goal in mind. Crs that only produce crs would make breeding higher quality crs easier. I would be upset if I was going for a certain patern but then it was a cbs and it be no use to me in that case. But if all the offspring are crs then you have higher chance of gets the desired pattern as well as color. I'm only 23 years old I don't care if it takes years I have time. Doing something your self and getting that end product is such a reward. I've taken a horible low grade red cherry colony into mostly fire sakura and painteds also I crossed those with blue velvets to get my own red rilli then bred those out till most of my colony is bbrr. This is just another project and in the end who knows maybe I'll have something really nice and just call it what I like. JamesHe 1 Quote Link to comment Share on other sites More sharing options...
JamesHe Posted May 26, 2015 Report Share Posted May 26, 2015 I always like to breed with a goal in mind. Crs that only produce crs would make breeding higher quality crs easier. I would be upset if I was going for a certain patern but then it was a cbs and it be no use to me in that case. But if all the offspring are crs then you have higher chance of gets the desired pattern as well as color. I'm only 23 years old I don't care if it takes years I have time. Doing something your self and getting that end product is such a reward. I've taken a horible low grade red cherry colony into mostly fire sakura and painteds also I crossed those with blue velvets to get my own red rilli then bred those out till most of my colony is bbrr. This is just another project and in the end who knows maybe I'll have something really nice and just call it what I like. Agree all you said. Quote Link to comment Share on other sites More sharing options...
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