AquaticShrimpNoob Posted November 17, 2018 Report Share Posted November 17, 2018 Hi Everyone, When I have started back in my aquarium hobby few years ago, I started to read about fish/aquarium care. This year as I get into shrimp keeping hobby, I started reading about post/threads/comments regarding about bacterias. I know that biofilm is probably the main food source of shrimps in the wild besides decaying organic matters. What struck me the most is the product bashing of people against bacteria based food for shrimps such as Bacter AE. Just to let it out there, I am not affiliated to the product Bacter AE. I am just a product user. As I read journal papers about the bacterias in Bacter AE (e.i. Bacillus subtitles and lactobacillus), I realized where this consumer issue (e.i. death) is coming from. For this topic, I will focus more on Bacillus subtitles. One instance caused of death that most people talked about is lacked of oxygen. I believe that this is true. Let's talk about some chemistry - Oxygen Solubility in Water (There are other sources for this if you want more accurate and trustworthy info. But I think that this will be sufficient.) Assume that your tank is at constant 70 F. Your tank's oxygen concentration is <10 mg/L according to this graph. According to this Dissolved Oxygen, the required oxygen concentration for bottom feeders (I am assuming shrimps are included here as well) is between 1-6 mg/L. This means that your tank is safe from oxygen starvation. Keep in mind that this oxygen-water solubility is not accounting pH, kH, gH and TDS - which all may affect oxygen-water solubility. Assuming everything is constant (e.i. temperature nearly does not fluctuate, water parameters do not change, and no over feeding) your shrimps and bacterias in your tank will be in harmony and thrive. Adding Bacter AE or other lived-bacteria containing products may destroy this if misuse. Bacillus subtitles is known as obligate aerobe, which means that they require oxygen to grow. In the case of Bacter AE, Bacillus subtitles will activate using oxygen. I have not found any study on this. But my guess is that the Bacillus subtitles will use up oxygen in the tank so fast than oxygen diffusing in water. This means that you have to initiate a means of enhancing mass transfer of oxygen to water by means of water agitation. In addition to oxygen absorption, there may be other factors that control the activation of Bacillus subtitles in water. For one is their food. I believe that oxygen absorption is for activation only, which means that you will only get X-times of live Bacillus subtitles because you only added X-times in your tank - who knows how many do activate. Growth and/or propagation is another issue. Bacillus subtitles has the potential to grow exponentially given that you have proper water conditions (e.g. pH and temperature) and source of carbon and nitrogen through decaying leaves, piece of drift wood, cholla, uneaten food, and nitrate. Therefore, a pinch amount of Bacter AE may be detrimental still for your tanks (some people did experienced this). And yes, the other ingredients included in Bacter AE (e.i. amino acids, polysaccharides, xylanase, glucanase, amylase, protease, and hemicellulase) can be food for the bacterias as well. On the other hand, I have seen people talked about Bacter AE causing death to their shrimps because the bacterias in it infected their shrimps. Assuming that Bacter AE contains Bacillus subtitles and Lactobacillus ONLY, this claim is "nearly" impossible. It is known that both of these species are probiotics and non-pathogenic. However, there is a study that reported Bacillus subtitles causing white spot syndrome to culture tiger shrimp Penaeus monsoon. I used to dose Bacter AE in my tank and never had any problem. In fact, this increased my baby shrimps' survival rate for sure. However, I started getting noticeable amount of rhabdocoela. Therefore, I stopped doing it. Now, I feed my own homemade (my own recipe) probiotic solution to my shrimps. I do not have any means of quantifying anything inside that solution. All I know are the reagents: water from my shrimp tank, table sugar, and Bacter AE. Using some journal papers that I found, I can speculate what by-products I can get in that solution. Thanks, AquaticShrimpNoob Revaria and rwong2k 2 Quote Link to comment Share on other sites More sharing options...
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